Amplified Fragment Length Polymorphism linkage ananysis of common buckwheat (Fagopyrum esculentum) and its wild self pollinating relative Fagopyrum homotropicum

Yasui¹, Yasuo, Yingjie Wang², Ohmi Ohnishi¹ and Clayton G. Campbell².

¹ Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Nakajo 1, Mozume-cho, Muko 617-0001, Japan² Kade Research Ltd., 135 13th Street, Morden, MB R6M 1E9, Canada

Yasui, Yasuo, Yingjie Wang, Ohmi Ohnishi and Clayton G. Campbell. 2004. Amplified Fragment Length Polymorphism linkage analysis of common buckwheat (Fagopyrum esculentum) and its wild self pollinating relative Fagopyrum homotropicum. Genome 47: 345-351.

Abstract: Common buckwheat (Fagopyrum esculentum) (2n = 2x =16) and Fagopyrum homotropicum (2n = 2x = 16) were mated in an interspecific cross and amplified fragment length polymorphism (AFLP) linkage maps were constructed by analyzing segregation in the F₂ population. Six hundred and sixty-nine bands were identified using 20 AFLP primer combinations, of which 462 (69%) segregated in the F₂ population. The map of F. esculentum has eight linkage groups with 223 markers covering a total of 508.3 cM. The map of F. homotropicum has eight linkage groups with 211 markers covering 548.9 cM. There was one to one correspondence of the esculentum and homotropicum linkage groups. Three morphological markers, distylous self-incompatibility, shattering habit and winged seed, were located on the AFLP map. Distylous self-incompatibility and shattering habit are tightly linked to each other (1.3 cM) and are located near the center of the linkage group 1. Winged seed is located on linkage group 4.

Key words: common buckwheat, AFLP, linkage analysis, distylous self-incompatibility, shattering habit.