Construction of genetic maps of common buckwheat (Fagopyrum esculentum Moench) and its wild relative, F. homotropicum Ohnishi based on Amplified Fragment Length Polymorphism (AFLP) markers

Yasuo Yasui¹, Yingjie Wang², Ohmi Ohnishi¹ and Clayton G. Campbell²

¹Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Kyoto, Japan ²Kade Research Ltd., Morden, Manitoba, Canada R6M 1E9

Yasuo Yasui, Yingjie Wang, Ohmi Ohnishi and Clayton G. Campbell. 2001. Construction of genetic maps of common buckwheat (Fagopyrum esculentum Moench) and its wild relative, F. homotropicum Ohnishi based on Amplified Fragment Length Polymorphism (AFLP) markers. Eighth International Symposium on Buckwheat, Chunchon, Korea, Kangwon University Press. Pp. 225-232.

Abstract: Genetic linkage maps of common buckwheat (F. esculentum) and F. homotropicum were constructed by AFLP markers segregating in an F₂ population of the interspecific cross F. esculentum x F. homotropicum. Of 672 bands generated by twenty AFLP primer combinations, 465 bands (69%) segregated in the F₂ population. The map of F. esculentum was comprised of eight linkage groups containing 224 markers and had a length of 508.3 cM. The map of F. homotropicum consisted of eight linkage groups with 214 markers covering 548.9 cM. One to one correspondence of the esculentum linkage groups to the homotropicum linkage groups was confirmed.

Key words: Buckwheat, F. homotropicum, F. esculentum, AFLP markers, linkage maps