Construction of a BAC Library of the Genus Fagopyrum

Mio Nagano¹, Jotaro Aii², Clayton Campbell³, Shinji Kawasaki⁴ and Taiji Adachi¹.

¹ Laboratory of Plant Genes and Physiology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka, 599-8531, Japan ² National Institute of Agrobiological Science, 2-1-2, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan (Japan Science and technology Corporation domestic Research Fellow) ³ Kade Research Ltd., 135 13 Street, Morden, Manitoba, Canada R6M 1E9. ⁴ National Institute of Agrobiological Science, 2-1-2, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan

Mio Nagano, Jotaro Aii, Clayton Campbell, Shinji Kawasaki and Taiji Adachi. 2001. Construction of a BAC Library of the Genus Fagopyrum. Eighth International Symposium on Buckwheat, Chunchon, Korea, Kangwon University Press. pp. 292-297.

Abstract: We have constructed a wild buckwheat (Fagopyrum homotropicum) bacterial chromosome (BAC) library. The library contains 24,096 clones stored in 251 96-well microtiter plates. A random sampling of 250 BACs indicted an average insert size of 97 kb. Based on a genome size of 1,080 Mkbp, the library coverage is 1.9 haploid genome equivalents. Screening the BAC library colony filters with cpDNA sequences showed that contamination of the genomic library with chloroplast clones was 11%. Library screening with a RFLP probe linked to the S-locus resulted in 2 hits. This library provides for isolation of the gene for the S-locus.

Key words: Buckwheat, Fagopyrum homotropicum, BAC Library