Characterization of AFLP markers linked to the S^h allele in buckwheat

Jotaro Aii¹, Mio Nagano², Masako Kuroda³, Clayton Campbell⁴ and Taiji Adachi²

¹ National Institute of Agrobiological Science, 2-1-2, Kannon-dai, Tsukuba, Ibaraki, 305-8602, Japan (Japan Science and technology Corporation domestic Research Fellow) ² Laboratory of Plant Genes and Physiology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka, 599-8531, Japan ³ Division of Applied Genetics and Biotechnology, Faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi 1-1, Miyazaki 889-2192, Japan ⁴Kade Research Ltd., 135 13 Street, Morden, Manitoba, Canada R6M 1E9.

Jotaro Aii, Mio Nagano, Masako Kuroda, Clayton Campbell and Taiji Adachi. 2001. Characterization of AFLP markers linked to the S^h allele in buckwheat. Eighth International Symposium on Buckwheat, Chunchon, Korea, Kangwon University Press. pp. 470-474.

Abstract: We have cloned and sequenced nine AFLP fragments closely linked to the S^h allele in buckwheat. In the nine, two AFLP fragments, N2 and N7, were derived from a single region in the buckwheat genome, especially N2 which was present in only F. homotropicum but was absent in common buckwheat, F. esculentum. These two AFLP fragments will facilitate marker assisted selection of the S^h allele and will be useful as genetic markers for fine mapping of the S locus in buckwheat.

Key words: buckwheat, F. esculentum, F. homotropicum, AFLP markers