Plant regeneration from cotyledon tissues of common buckwheat (Fagopyrum esculentum Moench.)

Sun Hee Woo¹, Taiji Adachi², Seung Keun Jong³ and Clayton G. Campbell⁴

¹ Agriculture and Agri-Food Canada, Crop Diversification Centre, Morden, Manitoba, Canada R6M 1Y5;² Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi 1-1, Miyazaki 889-2154, Japan; ³ Dept. of Agronomy, College of Agriculture, Chungbuk National University, Cheongju 360-763, Korea; ⁴ Kade Research Ltd. Morden, Manitoba., Canada R6M 1E9.

Woo, Sun Hee., Taiji Adachi., Seung Keun Jong and Clayton G. Campbell. 2000. Plant Regeneration from Cotyledon Tissues of Common Buckwheat (Fagopyrum esculentum Moench.). In Vitro Cell. Dev. Biol. Plant. 36: 358-361.

Abstract: Plants were regenerated from cotyledon tissues of greenhouse grown seedlings of common buckwheat (Fagopyrum esculentum Moench.). Maximum callus regeneration was induced on Murashige and Skoog (MS) medium containing 2,4-D (2.0 mg/l) and kinetin (KIN) (0.2 mg/l) and either 3 or 6% sucrose. Friable callus was transfered to MS media containing KIN and benzylaminopurine (BAP) at varied concentrations for embryonic callus induction. The optimum medium for embryonic callus induction was found to be MS medium supplemented with 0.2 mg/l KIN, 2.0 mg/l BAP and 3% (w/v) sucrose. Variation of sucrose from 3% to 6% did not show any significant effect on callus induction and embryogenesis. Regeneration of embryonic callus varied from 13 to 32%. Whole plants were obtained at high frequencies when the embryonic calli with somatic embryos and organized shoot primordia were transferred to half strength MS media with 3% sucrose. Regenerated plants after acclimation were transferred to greenhouse conditions, and both vegetative and floral characteristics were observed for variation. This regenerative system may be valuable for genetic transformation and cell selection in common buckwheat.

Key words: common buckwheat (Fagopyrum esculentum Moench.); cotyledon explant, plant regeneration, somatic embroygenesis.