Isolation of protoplasts from viable sperm cells of common buckwheat (Fagopyrum esculentum Moench.)

Sun Hee Woo¹, Taiji Adachi², Seung Keun Jong³ and Clayton G. Campbell⁴

¹ Agriculture and Agri-Food Canada, Crop Diversification Centre, Morden, Manitoba, Canada R6M 1Y5; ² Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi 1-1, Miyazaki 889-2154, Japan; ³ Dept. of Agronomy, College of Agriculture, Chungbuk National University, Cheongju 360-763, Korea; ⁴Kade Research Ltd. Morden, Manitoba, Canada R6M 1E9.

Woo, S. H. et al. 1999. Isolation of protoplasts from viable sperm cells of common buckwheat (Fagopyrum esculentum Moench.) Can. J. Plant Sci. 80: 583-585.

Abstract: Protoplasts from sperm cells were isolated. Sperm cells from germinated pollen, placed in solution were released, sieved and removed after sucrose density gradient centrifugation. The isolated cells were spherical and approximately 5.0-6.0 µm in diameter. Cytological observations confirmed that sperm cells of common buckwheat are true protoplasts.

Key words: common buckwheat (Fagopyrum esculentum Moench.), sperm cell isolation, osmotic shock.