Development of the SCAR markers linked to the S^h gene in buckwheat

Aii, Jotaro ¹, Mio Nagano ¹, Sun Hee Woo ² and Clayton Campbell ²

¹ Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi, Miyazaki 889-2155, Japan, ² Kade Research Ltd. 135 13 Street, Morden, Manitoba, R6M 1E9, Canada.

Aii, Jotaro, Mio Nagano, Sun Hee Woo and Clayton Campbell. 1999. Development of the SCAR markers linked to the S^h gene in buckwheat. Fagopyrum 16: 19-22.

Abstract: Three RAPD markers, OPB14₁₂₅₀, OPP8₁₀₀₀ and OPQ7₈₀₀, tightly linked to S^h gene were cloned and characterized. Nucleotide sequence information, obtained from each end of the three linked RAPD markers, was used to design 21-mer oligonucleotide primers for PCR amplification of the respective SCARs. Two of these SCAR primers, OPB14₁₂₅₀, OPP8₁₀₀₀, amplified a single fragment in Fagopyrum homotropicum but was absent in common buckwheat, F. esculentum. In one case, SCQ7₈₀₀ showed different size PCR fragments in common buckwheat than in F. homotropicum. This co-dominant marker is useful for differentiating heterozygosity from both types of homozygote.

Key words: buckwheat, Fagopyrum esculentum, SCAR, markers, self-compatibility