Development of the Amplified Fragment Length polymorphisms (AFLP) in Buckwheat

Nagano, Mio¹, Jotaro Aii¹, Clayton Campbell² and Taiji Adachi¹.

¹ Division of Applied Genetics and Biotechnology, Faculty of Agriculture, Miyazaki University, Miyazaki 889-2192, Japan ² Kade Research Ltd., Morden, Manitoba, Canada R6M 1E9

Nagano, Mio, Jotaro Aii, Clayton Campbell and Taiji Adachi. 1998. Development of the Amplified Fragment Length Polymorphisms (AFLP) in Buckwheat. Advances in Buckwheat Research. Seventh International Symposium on Buckwheat, Winnipeg, Manitoba Aug. 12-14. University of Manitoba Press. pp. V: 58-64

Abstract: Common buckwheat, Fagopyrum esculentum, is a heteromorphic sporophytic incompatibility species while an annual wild species, F. homotropicum is homomorphic self-comptible. An F₂ population was generated from an interspcfic hybrid between them. We used AFLP and bulked segregate analysis to develop molecular markers linked to the self compatibility (S^h) gene. AFLP provides many genomic bands for buckwheat. 62 bands specific to homomrphic flowered plants were detected. AFLP should be useful for marker assissted selection for introgession of self-compatibility into common buckwheat and also for detailed analysis of plant genomes.

Key words: buckwheat, F. esculentum, F. homotropicum, AFLP, homomorphism