Kade Research Ltd.
Abstract
Molecular marker linked to the S locus in buckwheat
Aii, Jotaro1, Mio Nagano1, Sun Hee Woo1, Clayton Campbell2 and Taiji Adachi1.
1 Division of Applied Genetics and Biotechnology, Faculty of Agriculture, Miyazaki University, Miyazaki 889-2192, Japan 2 Kade Research Ltd., Morden, Manitoba, Canada R6M 1E9
Aii, Jotaro, Mio Nagano, Sun Hee Woo, Clayton Campbell and Taiji Adachi. 1998. Molecular marker linked to the S locus in buckwheat. Seventh International Symposium on Buckwheat, Winnipeg, Manitoba Aug. 12-14. University of Manitoba Press. pp. V: 65-71.
Abstract:We have cloned and sequenced three RAPD fragments closely linked to the Sh gene in buckwheat. Nucleotide sequence information, obtained from each end of the three linked RAPD markers, was used to design 21-mer olignucleutide primers for PCR amplification of the respective SCARs. Two of these SCAR primers, SCB141250 and SCP81000 amplified a single fragment in Fagopyrum homotropicum but was absent in common buckwheat, F. esculentum. In one case SCQ7800, showed different PCR fragments between common buckwheat and F. hmotropicum. This co-dominant marker would be useful for differentiating between heterozygous and both types of homozygous forms.
Key words: buckwheat, F. esculentum, F. homotropicum, AFLP, PCR, RAPD, homomorphism
