Kade Research Ltd.
Abstract
Development of the SCAR markers linked to the Ho gene in buckwheat.
Aii, J.1, Nagano, M.1, Campbell C.2 and Adachi, T3.
1 Applied Genetics and Biotechnology Division, faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi 1-1, Miyazaki 889-2192, Japan. 2 Kade Research Ltd. 135-13 Street, Morden, Manitoba, R6M 1E9, Canada. 3 Laboratory of Plant Genes and Physiology, Graduate School of Agriculture and Biological Sciences, Osaka Prefectural University, Gakuen-Cho, Sakai, Osaka, 599-8531, Japan.
Aii, J., Nagano, M., Campbell, C. and Adachi, T. Development of the SCAR markers linked to the Ho gene in buckwheat. 1997. Breed. Sci. (Suppl. 2): 198.
Abstract: We have cloned and sequenced three RAPD markers tightly linked to the Ho gene in buckwheat. We have also developed SCAR markers based on their sequence information. Two of these SCAR markers amplified as a single fragment in Fagopyrum homotropicum but were absent in F. esculentum. One of these marker showed different PCR fragments between F. homotoropicum and F. esculentum. This co-dominant marker could be useful for differentiating between heterozygous and homozygous forms.
Key words: AFLP analysis, Fagopyrum esculentum, buckwheat, DNA
